Size-exclusion chromatography of xylan derivatives—the critical evaluation of macromolecular data

Size Exclusion Chromatography of Polymers

In the characterization of polymers, size-exclusion chromatography (SEC) has become a standard technique for determining molar mass averages andmolar mass distributions (MMD) of polymers. The principle of SEC is easily understood: owing to limited accessability of the pore volume within the particles of the column packing, polymer molecules are separated according to their hydrodynamic volumes,...

Evaluation of Models for Size Exclusion Chromatography of Asymmetric Solutes

We consider two presentations regarding the size exclusion chromatography of highly asymmetric solutes: one is embodied in the statistical model of Giddings et al.;I the second is described by the concept of "end-on insertion" proposed by Tanford* and expanded upon by Meredith and N a t h a n ~ . ~ Our evaluation is based on measurements of Kfor the rigid rodlike and spherical solutes schizophy...

Measurement of Albuminuria with Size-exclusion Chromatography

Size exclusion chromatography does not require pores.

Separation of macromolecules on the basis of their molecular weight by size exclusion chromatography has long been considered to be caused by the geometry-dependent partition of macromolecules between a continuous phase and the porous interior of a gel or cross-linked bead. The volume of a pore accessible to a solute is limited by its relative dimensions, so larger molecules will have access to...

Size-exclusion chromatography using core-shell particles.

Size-exclusion chromatography (SEC) is an indispensable technique for the separation of high-molecular-weight analytes and for determining molar-mass distributions. The potential application of SEC as second-dimension separation in comprehensive two-dimensional liquid chromatography demands very short analysis times. Liquid chromatography benefits from the advent of highly efficient core-shell ...